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pCAG-MBP-ATG9-V836A

Plasmid

#188080

Purpose

Expresses human ATG9-V836A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

V836A

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-H837A

Plasmid

#188079

Purpose

Expresses human ATG9-H837A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

H837A

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (1-830)

Plasmid

#188086

Purpose

Expresses human ATG9 C-terminal truncation mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (1-830)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-P833A

Plasmid

#188083

Purpose

Expresses human ATG9-P833A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

P833A

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

Plasmid

#186414

Purpose

AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli)

Use

Gateway destination vector

Tags

Nuclear localization signal

Expression

Mutation

Promoter

Basal promoter of the FOG gene

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GB1-EWS_171-264

Plasmid

#180466

Purpose

Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GB1 and His8

Expression

Bacterial

Mutation

deleted 1-170, 265-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GFP-EWS_1-120

Plasmid

#180464

Purpose

Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GFP and His8

Expression

Bacterial

Mutation

deleted 121f-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB1

Plasmid

#180209

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-409

Promoter

AdhI

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-RHD

Plasmid

#180207

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-581

Promoter

AdhI

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB1

Plasmid

#180200

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot8-D40AE42A_U

Plasmid

#147647

Purpose

Mammalian Expression of HsNot8-D40AE42A

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8-D40AE42A (CNOT8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

Plasmid

#172582

Purpose

For transient expression of C-terminal BirA-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB2

Plasmid

#180204

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Use

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB1

Plasmid

#180203

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Use

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Use

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1351-1588_AE

Plasmid

#148630

Purpose

Bacterial Expression of HsNot1iso1_1351-1588

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1351-1588 (CNOT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_141-442_AG

Plasmid

#148833

Purpose

Bacterial Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4-del190-274_AG

Plasmid

#148782

Purpose

Mammalian Expression of HsNot4-del190-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del190-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_13-240_U

Plasmid

#147649

Purpose

Bacterial Expression of HsNot8_13-240

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_13-240 (CNOT8 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-Cterm

Plasmid

#180208

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 542-1224

Promoter

AdhI

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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