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Plasmid#179901PurposeDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorInsertOxysterol-binding Protein PH domain (OSBP Human)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-TVA66T-EGFP
Plasmid#64091PurposeExpresses TVA66T-EGFP under the CAG promotorDepositorInsertTVA66T-EGFP
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationGlutamic acid 66 to ThreoninePromoterCAGAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ELYS-emerin-EGFP-polyA
Plasmid#59745PurposeExpress ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertELYS-emerin-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJEP204-Lenti-TRE3G-GFP-WPRE-Pa
Plasmid#164788PurposepLenti vector backbone designed to express GFP from a TRE3G promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter1.3αCaMKIIAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn2a-HaloTag-V5-EF1-sfGFP
Plasmid#182564PurposeFor mouse Nav1.2 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn2a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
hMYOG-3xHA-IRES-hrGFP (#324)
Plasmid#78341PurposeOverexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorInsertMYOG (MYOG Human)
UseTags3x HA tag and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FLEX-rc[Chronos-GFP]
Plasmid#62725PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1αAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mouse PKA-Calpha-mEGFP
Plasmid#45528DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseWith chicken beta actin promoter and wpreTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 (aka VD1)
Plasmid#46270DepositorInsertVcl 1-258 (aka VD1) (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-258 (VD1 domain)PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRB-GFP-Gab2(Y604F/Y634F)
Plasmid#188659PurposeFRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to PheDepositorInsertGRB associated binding protein 2 (Gab2 Mouse)
UseTagsFRB and GFPExpressionMammalianMutationY604F and Y634FPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B2-GFP
Plasmid#123109PurposeExpresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP
Plasmid#154303PurposeExpresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideumDepositorInsertshistone H1
γ-tubulin
UseDictyostelium expressionTagsmEGFP and mRuby3ExpressionMutationPromoteractin15Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLF091: Lenti-CaMKIIa-eTsChR-eGFP
Plasmid#112281PurposeFor lentiviral or direct expression of eTsChR in excitatory neuronsDepositorInserteTsChR
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2 GFP-P2A-BFP
Plasmid#202195Purposeknock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP-P2A-BFP knock-inExpressionMutationPromoterlacAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-GFP microRNA-10b
Plasmid#16070DepositorInsertmicroRNA-10b (MIR10B Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
12_pAAV-ProC2-CatCh-GFP-WPRE
Plasmid#125938PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC2Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-NES-mut
Plasmid#42001DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationF951A, F955A, I958A, F960APromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
221-RPB1-AID-eGFP-2A-blasticidin
Plasmid#195107PurposeTargeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertAID-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
KI(GAPDH)-P2A-EGFP-CMV-NeoR
Plasmid#114009PurposeHDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selectionDepositorInsertsUseTagsExpressionMammalianMutationPromoter-, CMV, and endogenous promoter (chicken GAPDH in…Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only