Addgene

Showing: 10621 - 10640 of 52622 results

Results per page:

Sort results by:

pDONR221_SLC9A4

Plasmid

#132246

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A4 (SLC9A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-His10TEV

Plasmid

#159427

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Use

Tags

TEV-protease-cleavable His10-tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC16A7

Plasmid

#131947

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A7 (SLC16A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

Plasmid

#189955

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

Plasmid

#102593

Purpose

Lentivector to express STING V155M S366A and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M S366A

Promoter

SFFV

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

intein/chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-6xHis-SUMO1

Plasmid

#164939

Purpose

Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Small Ubiquitin Like Modifier 1 (SUMO1 Human)

Use

Lentiviral

Tags

6XHIS

Expression

Mutation

Promoter

CMV

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC6A9

Plasmid

#132220

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A9 (SLC6A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HNF4A_P2-257

Plasmid

#31063

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-257 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC26A9

Plasmid

#132140

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A9 (SLC26A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LoxP3-HA-H2B-Cerulean-2A (SO104)

Plasmid

#99618

Purpose

To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC25A10

Plasmid

#132014

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A10 (SLC25A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC25A18

Plasmid

#132003

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A18 (SLC25A18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HNF4A_P2-164

Plasmid

#31066

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-164 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_MECP2_MBD-R106W

Plasmid

#179408

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R106W) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R106W

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge