-
Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC-mTRF1
Plasmid#64162PurposeRetroviral vector expressing mouse TRF1 with N-terminal Myc tagDepositorInsertmTRF1 (Terf1 Mouse)
UseRetroviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PHD1
Plasmid#21400DepositorInsertEGLN2 (EGLN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MLC2(CA)-IRES-GFP
Plasmid#133928PurposeBicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chainDepositorInsertEGFP and MLC2(CA) (MYL2 Human)
UseTagsExpressionMammalianMutationMLC2 T18D, S19DPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
H1.M11.luc
Plasmid#193707PurposeExpressed a H1-core mutant by introducing multiple 7SK elements (octamer, PSE, spacer, and TATA box)DepositorInsertMutated H1-core promoter with multiple 7SK elements (octamer, PSE, spacer, and TATA box)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgMTHFD2_1
Plasmid#106313PurposeExpress Cas9 and sgRNA targeting MTHFD2DepositorInsertsgRNA targeting MTHFD2 (MTHFD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
Plasmid#162333PurposeLentiviral expression of dSpCas9-2xVP64 with blasticidin resistanceDepositorInsertdSpCas9-2xVP64
UseLentiviralTagsFLAGExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_IRES-blast
Plasmid#166411Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationPromoteref-1aAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a-EGFP
Plasmid#205311PurposeExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a-EGFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV Flag PAI-RBP
Plasmid#45452DepositorInsertPAI-RBP (SERBP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationPromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TBX5-FLAG in pLX303
Plasmid#42563DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Human HMG-CoA lyase like 1 (HMGCLL1)
Plasmid#52434Purposemammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)DepositorInsertHMGCLL1 (HMGCLL1 Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTag-RFP-C-h-Rab7L1-c-Myc
Plasmid#79804PurposeExpress TagRFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagRFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only