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pDONR223-DAPK1

Plasmid

#23480

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAPK1 (DAPK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-SEAP

Plasmid

#24595

Depositor

Alan Cochrane

Insert

SEAP (ALPP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-S100A10

Plasmid

#107200

Purpose

Expresses YFP-human S100A10 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Insert

S100A10 (S100A10 Human)

Use

Tags

Yellow Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSH231-Bx-GFP-C31

Plasmid

#115149

Purpose

Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Safe Harbor Homology Sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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4xmts-mScarlet-I

Plasmid

#98818

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

mts

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAGM9121

Plasmid

#51833

Purpose

level 0 Universal acceptor

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225246

Purpose

Mammalian expression of iPASnFR-L in the nucleus

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-mDlx-jGCaMP8f-WPRE

Plasmid

#176753

Purpose

jGCaMP8f expression in GABA-ergic interneurons under the control of mDlx enhancer

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pACUH

Plasmid

#58374

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Yuh-Nung Jan, Chun Han

Article

Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

Plasmid

#127392

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid and Threon…

Promoter

pCAG

Available since

July 3, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA5-Halo

Plasmid

#175326

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA5 (HSPA5 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Mutation

Promoter

Chick beta actin pomoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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