-
Plasmid#205118PurposeRHOA protein expressionDepositorInsertRHOA (RHOA Human)
UseTags6His-TEV and AviTagExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-CV-B3_3C
Plasmid#203499PurposeExpresses CV-B3 3C protease from a GAL promoter with a URA3 markerDepositorInsertCV-B3 3C protease (D1P32_gp1 )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
iB22 PB-zim3-mycNLS-mApple
Plasmid#204718PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-SV-A_3C
Plasmid#203474PurposeExpresses SV-A 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertSV-A 3C protease (Hk1_gp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HPeV_3C
Plasmid#203465PurposeExpresses HPeV 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHPeV 3C protease (polyprotein gene )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PATL2_G288-Y543_BV_his
Plasmid#194760DepositorInsertPATL2 (PATL2 Human)
UseBaculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Twist3cterm-GFPnls-T2A-ER-Cre-ER
Plasmid#200105DepositorInsertTwist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI_mini-NtA-Fs1-mSA-Myc-His
Plasmid#198136PurposeExpress NtA-Fs1 in mammalian cellsDepositorInsertNtA-Fs1
UseTagsmSA, TEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
pCCL-c-MNDU3-CD3D A0 cDNA
Plasmid#198021PurposeLV Expression of CD3D cDNA with mutation at c.209T>C to assess a bystander editDepositorInsertCD3D A0 cDNA
UseLentiviralTagsExpressionMammalianMutationc.209T>C to assess a bystander editPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RENBP_11-427_BioN
Plasmid#195869PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRENBP (RENBP Human)
UsePfbd-bira, baculovirus expressionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNot1_1499-1644_R
Plasmid#147355PurposeBacterial Expression of DmNot1_1499-1644DepositorInsertDmNot1_1499-1644 (Not1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmNot1_1499-1644_R
Plasmid#147357PurposeBacterial Expression of DmNot1_1499-1644DepositorInsertDmNot1_1499-1644 (Not1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-deltaUBA-short-UBASH3A
Plasmid#195405PurposeMammalian expression of human short-form UBASH3A lacking the UBA domain with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
K40R-short-UBASH3A-V5-6xHis
Plasmid#195407PurposeMammalian expression of human K40R short-form UBASH3A with V5-6xHis tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5-6xHisExpressionMammalianMutationK40RPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGB-06-07-Vps34
Plasmid#187989PurposePlasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323DepositorInsertVps34 (PIK3C3 Human)
UseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K202R-long-UBASH3A-Ntag
Plasmid#192093PurposeHuman K202R long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationK202RPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmNot1_1147-1717_P
Plasmid#147187PurposeBacterial Expression of DmNot1_1147-1717DepositorInsertDmNot1_1147-1717 (Not1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-DmNot1_1147-1717_N
Plasmid#147018PurposeBacterial Expression of DmNot1_1147-1717DepositorInsertDmNot1_1147-1717 (Not1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only