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Showing: 5291 - 5300 of 152043 results

  2. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  6. pRKO

    Plasmid
    #49456
    Purpose
    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
    Depositor
    Marc Gartenberg
    Article
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    loxP-kanMX-loxP cassette
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49456-plasmid-map-sequence-id-73667

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  7. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF

    Plasmid
    #163317
    Purpose
    Encodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase
    Depositor
    Dawn Chandler
    Insert
    Nsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Luciferase
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    163317-plasmid-map-sequence-id-328584

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  9. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Use
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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Showing: 5291 - 5300 of 152043 results