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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

Plasmid

#195741

Purpose

Base editing for making a pre-stop codon in mmoX using phenol inducible system

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Po (phoenol-inducible promoter)

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194284

Purpose

EF1a-dCas9-KRAB-GFP with nontarget sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGW03 AAV-TBG-Cre-sgRNA

Plasmid

#192163

Purpose

AAV-TBG-Cre-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(CTRL)-CMV-eGFP

Plasmid

#194017

Purpose

Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(CTRL)
eGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLL1 sgRNA(MS2)-MS2-AIDmono

Plasmid

#112127

Purpose

empty sgRNA cloning vector with MS2-AIDmono

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, H130A/R131E…

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_zeo insert c-MYC

Plasmid

#164636

Purpose

sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

Depositor

Günter Schneider

Insert

target sequence for c-Myc activation (MYC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1A

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO

Available since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3 sgRNA(MS2)-MS2-AID3f

Plasmid

#112129

Purpose

empty sgRNA cloning vector with MS2-AID3f

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AIDmono 3f mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLL2 sgRNA(MS2)-MS2-AID3c

Plasmid

#112128

Purpose

empty sgRNA cloning vector with MS2-AID3c

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono 3c mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti pTERT sgRNA1(MS2) mCherry

Plasmid

#171054

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA1(MS2) mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLL4 sgRNA(MS2)-MS2-hA3a

Plasmid

#112130

Purpose

empty sgRNA cloning vector with MS2-humanA3a

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a (APOBEC3A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

Plasmid

#181792

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC

Use

Tags

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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