Skip to main content
Addgene
Showing: 1041 - 1060 of 15711 results

  1. pLIX403-EBFP2-ccdB-Puro

    Plasmid
    #158552
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158552-plasmid-map-sequence-id-309842

    Enlarge

  2. LZRS-Mek2-wt (2-3)

    Plasmid
    #21201
    Depositor
    Paul Khavari
    Insert
    Mek2 (MAP2K2 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  3. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

    Enlarge

  4. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

    Enlarge

  5. pLJM60

    Plasmid
    #34610
    Depositor
    David Sabatini
    Insert
    deptor (DEPTOR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

    Enlarge

  7. pBabepuro-IL8

    Plasmid
    #189608
    Purpose
    Retroviral expression vector for IL8
    Depositor
    Thierry Tchenio
    Insert
    IL8 (CXCL8 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    SV40 early promoter
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189608-plasmid-map-sequence-id-381193

    Enlarge

  8. pX459-puro-hPKAalpha

    Plasmid
    #185379
    Purpose
    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185379-plasmid-map-sequence-id-363450

    Enlarge

  9. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-alpha
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

    Enlarge

  10. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid
    #72258
    Purpose
    Express FLPe, copGFP and Puromycin resistance gene under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-copGFp-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72258-plasmid-map-sequence-id-134986

    Enlarge

  11. pLV_Zika_Flag_NS1 (Variant: W98G)

    Plasmid
    #79633
    Purpose
    Third generation Self-inactivating lentivector expressing NS1 (Variant W98G) from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS1
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Variant: W98G
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79633-plasmid-map-sequence-id-148801

    Enlarge

  12. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

    Enlarge

  13. pBABE.puro/TO/Flag/mKO2

    Plasmid
    #110353
    Purpose
    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    monomeric Kusabira Orange 2
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110353-plasmid-map-sequence-id-254685

    Enlarge

  14. pBABEpuro-ARAF_S214T

    Plasmid
    #51079
    Purpose
    mammalian expression of ARAF S214T
    Depositor
    Matthew Meyerson
    Insert
    ARAF (ARAF Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    S214T
    Promoter
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51079-plasmid-map-sequence-id-77345

    Enlarge

  15. pGH643

    Plasmid
    #230834
    Purpose
    hu6-BsmBI-MS2_sgRNA-Ef1a-puroR-T2A-mCherry-wPRE
    Depositor
    Gaelen Hess
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    230834-plasmid-map-sequence-id-458481

    Enlarge

  16. pMSCV-Hipk1

    Plasmid
    #221508
    Purpose
    Retrovirus-mediated gene transfer into mammalian cells to overexpress Hipk1
    Depositor
    Alessandro Ori
    Insert
    Hipk1 (Hipk1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221508-plasmid-map-sequence-id-439445

    Enlarge

  17. LT3GEPIR-MSH2shRNA1

    Plasmid
    #220587
    Purpose
    To inducibly knockdown MSH2 expression
    Depositor
    Sean Lee
    Insert
    MSH2 shRNA1 (MSH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220587-plasmid-map-sequence-id-437724

    Enlarge

  18. pGS96_EGFP(A206K)-I3-01 AAVS donor

    Plasmid
    #211865
    Purpose
    I3-01 transgene integration at AAVS1 safe harbor locus
    Depositor
    Iain Cheeseman
    Insert
    I3-01
    Use
    CRISPR
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211865-plasmid-map-sequence-id-426339

    Enlarge

  19. pcDNA3.1-Pur-BFP-P2A-ppViperin

    Plasmid
    #217483
    Purpose
    Expression of Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmon
    Depositor
    Bertrand Collet
    Insert
    BFP-P2A-otPKRSL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217483-plasmid-map-sequence-id-430350

    Enlarge

  20. pLentiCRISPR-v2 sgUCKL1

    Plasmid
    #211523
    Purpose
    Deletes UCKL1
    Depositor
    Richard Possemato
    Insert
    sgUCKL1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    211523-plasmid-map-sequence-id-418645

    Enlarge

Showing: 1041 - 1060 of 15711 results