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Plasmid#200342Purposegfp BioPart for PVP neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVPp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003840, ocr-3Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH78 - [AWBp | gfp | tbb-2 UTR]
Plasmid#200352Purposegfp BioPart for AWB neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWBp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00005701, sru-38Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH76 - [ADFp | gfp | tbb-2 UTR]
Plasmid#200350Purposegfp BioPart for ADF neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[ADFp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00005359, srh-142Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH60 - [ASELp | gfp | tbb-2 UTR]
Plasmid#200334Purposegfp BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[ASELp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001533, gcy-6Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-12xmut_1-1115_P
Plasmid#147159PurposeInsect Expression of DmGW182-12xmut_1-1115DepositorInsertDmGW182-12xmut_1-1115 (gw Fly)
UseTagsExpressionInsectMutationone silent and two non silent mutations V704A, N…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-830-dsRNAres_N
Plasmid#146972PurposeInsect Expression of DmGW182_1-830-dsRNAresDepositorInsertDmGW182_1-830-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1115-dsRNAres_N
Plasmid#146973PurposeInsect Expression of DmGW182_1-1115-dsRNAresDepositorInsertDmGW182_1-1115-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationtwo non silent mutations V704A and N799S, and one…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM2-dsRNAres_H
Plasmid#146438PurposeInsect Expression of DmGW182-delM2-dsRNAresDepositorInsertDmGW182-delM2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1085-1605-del1_V
Plasmid#147750PurposeMammalian Expression of HsNot1iso1_1085-1605-del1DepositorInsertHsNot1iso1_1085-1605-del1 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1085-1605-del2_V
Plasmid#147751PurposeMammalian Expression of HsNot1iso1_1085-1605-del2DepositorInsertHsNot1iso1_1085-1605-del2 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1085-1605-del3_V
Plasmid#147752PurposeMammalian Expression of HsNot1iso1_1085-1605-del3DepositorInsertHsNot1iso1_1085-1605-del3 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP
Plasmid#196989PurposeDerived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNADepositorInsertNon-Target
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct
Plasmid#86230PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#92140PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvpu/env
Plasmid#101345PurposeDeletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RREDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vpu/EnvPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvif/vpr
Plasmid#101344PurposeDeletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequenceDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vif/VprPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-del57-499_H
Plasmid#146460PurposeInsect Expression of DmHPat-del57-499DepositorInsertDmHPat-del57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation C2340A compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only