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Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:FCP:ShBle
Plasmid#85985PurposeExpresses ShBle with FCP promoter/terminator for F. cylindrus transformation.DepositorInsertShBle
UseGolden gate assemblyTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD708 DsRed-Express2-ZF21
Plasmid#138795PurposeConstitutive expression of DsRed-Express2-ZF21 under the CMV promoter.DepositorInsertDsRed-Express2-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rich-pT7GG
Plasmid#108443PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assemblyDepositorTypeEmpty backboneUseXenopus oocyte expression systemTagsExpressionMutationPromoterT7Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
C64m
Plasmid#120980PurposeMoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).DepositorInserttemperature sensitive lambda repressor (cI857)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C35m
Plasmid#120969PurposeMoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).DepositorInsertRhlR
UseSynthetic BiologyTagsExpressionMutationBsaI and BbsI sites removed; amino acid sequence …PromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD706 DsRed-Express2-ZF13
Plasmid#138793PurposeConstitutive expression of DsRed-Express2-ZF13 under the CMV promoter.DepositorInsertDsRed-Express2-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
UC20m
Plasmid#121040PurposeMoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).DepositorInsertgN2 Cas9 gRNA UC part
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C71m
Plasmid#120983PurposeMoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertCinR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
L1B42-pGATA23-Bxb1-SAUR-tUBQ10
Plasmid#219705PurposeLevel 1 pGATA23-Bxb1-SAUR-tUBQ10, for lateral root specific expression of Bxb1 integrase with SAUR tagDepositorInsertpGATA23:Bxb1:SAUR:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1426
Plasmid#218622PurposeExpress tNCS to the peroxisome for toxicity assayDepositorInserttNCS
UseTagsYFP-ePTS1ExpressionYeastMutation106-588PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1465
Plasmid#218625PurposeExpress engineered TFs Adr1c(S230A), Pip2c, and Oaf1cDepositorInsertAdr1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1734
Plasmid#218627PurposeExpress amorphadiene synthase (ADS)DepositorInsertADS
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1535
Plasmid#218613PurposeExpress Pex5DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1401
Plasmid#218615PurposeExpress Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12DepositorInsertPex13
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1536
Plasmid#218617PurposeExpress Pex5 and Pex11DepositorInsertPex5
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1469
Plasmid#218588PurposeExpress peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonateDepositorInsertMvaS
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg
Plasmid#222826PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan
Plasmid#222827PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only