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Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKL002
Plasmid#98921PurposepET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.DepositorInsertsPROPS
GCaMP6f
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL003
Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
UseTagsHisExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon
Plasmid#69906PurposeExpresses the human ciRS7/CDR1as circular RNA in mammalian cellsDepositorInsertciRS7 (CDR1-AS Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH75
Plasmid#128224Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
UseTagsHis and mRuby3ExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNox4
Plasmid#69353Purposeexpresses mouse Nox4 in mammalian cellsDepositorInsertmNox4 (Nox4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNoxo1
Plasmid#58530Purposeexpresses human Noxo1 in mammalian cellsDepositorInsertNADPH oxidase organizer 1 (NOXO1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PBD-EYFP
Plasmid#13723DepositorInsertPBD-EYFP (PAK1 Human)
UseTagsEYFPExpressionMammalianMutationp21 binding domain of human Pak1(amino acids 65-1…PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse NCKX3 (pcDNA3.1+)
Plasmid#75204PurposeMammalian expression of SLC24A3DepositorInsertSLC24A3 (Slc24a3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH113
Plasmid#128210Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNox1
Plasmid#58340Purposeexpresses mouse Nox1 in mammalian cellsDepositorInsertMus musculus NADPH oxidase 1 alpha (Nox1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH49
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH51
Plasmid#128213Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-WDR5 shRNA 2
Plasmid#59975PurposeKnockdown of WDR5DepositorInsertWDR5 shRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only