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Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorInsertNucleotide diphosphate kinase (ndk Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME pHluorin2
Plasmid#73794Purposeratiometric pHluorin2 sequence in a middle entry cloneDepositorInsertratiometric pHluorin2
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAYP (pDre)
Plasmid#62709PurposeMammalian expression of Dre recombinase from the broadly active CAG promoter/enhancerDepositorInsertDre
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD33-Gm
Plasmid#65098Purposebacterial expression vector with Gentamycin selection, based on pBAD33DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::ZeoR-bGHpA
Plasmid#62442PurposeMXS_chaining vector with PGK::ZeoR-bGHpADepositorInsertresistance cassette against Zeocin with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-GFP
Plasmid#83375PurposeExpression of Myc tagged GFPDepositorInsertMyc-GFP
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgDonor_lentiGuide
Plasmid#184168PurposeLentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.DepositorInsertsgDonor
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
APEX2-ChBP
Plasmid#108878PurposeExpression of APEX2 fused to a nanobody against mCherryDepositorInsertAPEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRU41
Plasmid#167663PurposeENTRY vector for gRNA1 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-gTEMP_pcDNA3
Plasmid#109117PurposeMitochondria targetting gTEMPDepositorInsertmito-gTEMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU42
Plasmid#167664PurposeENTRY vector for gRNA2 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
gTEMP_pcDNA3
Plasmid#89583PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertgTEMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control
Plasmid#128749PurposeExpresses control gRNADepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly
Plasmid#71499PurposeflySTARR-seq screening vector with DSCP core promoter.DepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only