-
Plasmid#119983PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIR-R/Tac1/SG
Plasmid#16296DepositorInsertTac1 3'UTR (TAC1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pNCMV 16E6no* Y54D
Plasmid#47705PurposeDefective for p53 bindingDepositorInsertHPV 16 E6no* (E6 HPV)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7 aa,Y54D, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
deltaCT S6K1 MSCV puro
Plasmid#8465DepositorInsertdelta ct p70 S6K1 (Rps6kb1 Rat)
UseRetroviralTagsmycExpressionMammalianMutationdeletion of 104 c-terminal amino acidsPromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-08
Plasmid#119987PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1E(sfGFP)
Plasmid#126982PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SaCas9_sgRNA2
Plasmid#107330PurposeU6 driven SaCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LacD_WT_pET21a
Plasmid#29785DepositorInsertTagatose 1,6-diphosphate aldolase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
LacD_L165E_pET21a
Plasmid#29786DepositorInsertTagatose 1,6-diphosphate aldolase
UseTagsHisExpressionBacterialMutationL165EPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
LacD_L275S_pET21a
Plasmid#29787DepositorInsertTagatose 1,6-diphosphate aldolase
UseTagsHisExpressionBacterialMutationL275SPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95PDZGW (C6)
Plasmid#74022Purposelentiviral expression of Psd95 shRNA and Psd95-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationPDZPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pANY3
Plasmid#112197PurposeThis plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK581] pRK5 HA-SLC38A9N
Plasmid#136141Purposemammalian expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Adeno-miR-27b
Plasmid#112539PurposeAdenovirus overexpressionDepositorInsertmiR-27b (MIR27B Human)
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xRor2-EGFP
Plasmid#67163Purposemammalian expression / in vitro transcriptionDepositorInsertTyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
UseIn vitro transcriptionTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYF-2xGfaABC1D-tTA
Plasmid#19981DepositorInsert2xGfaABC1D-tTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C1
Plasmid#50353PurposeMouse eIF2C1 cDNA cloneDepositorInserteIF2C1 (Eif2c1 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only