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JAB2657

Plasmid

#190393

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::LacI-NLS-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::LacI-NLS-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2618

Plasmid

#190391

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::LacI-VP16-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::LacI-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2337

Plasmid

#190367

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-ERF2(AD)-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-ERF2(AD)-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2283

Plasmid

#190364

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ZAT10(RD)-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ZAT10(RD)-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2165

Plasmid

#190348

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-VP16-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::QacR-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2164

Plasmid

#190347

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::LmrA-VP16-19St)

Depositor

Jose Dinneny, Jennifer Brophy

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::LmrA-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Use

Tags

eGFP

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-Num1-PH

Plasmid

#71369

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Use

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

tac

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Use

Tags

3XFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25c

Plasmid

#168013

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25b

Plasmid

#168012

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMB-PDESr

Plasmid

#61207

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB501NL3F10H

Plasmid

#141286

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Kv-ArcLight-ST

Plasmid

#182319

Purpose

Soma-targeted fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Article

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

Kv-ArcLight-ST

Use

Tags

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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YEp112-CUP1-His-Smt3

Plasmid

#99537

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-Skm1-PH

Plasmid

#71371

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Skm1-PH

Use

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa1-132

Promoter

tac

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
HERC2 (3KCI)

Plasmid

#25344

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

herc2.3951.4321 (HERC2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 3951-4321

Promoter

Available since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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