-
Plasmid#153947PurposepiggyBac transposon vector with CAG promoter expressing Otx2 and EGFPDepositorInsertOtx2 (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEY43
Plasmid#191047Purposeeat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInserteat-4(prom6-1) (eat-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAT2B
Plasmid#34795DepositorInsertmethionine adenosyltransferase II, beta (MAT2B Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Axin2-luciferase
Plasmid#21275DepositorInsertAxin2 promoter (Axin2 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
SNX5-PX (26-170)
Plasmid#119086PurposeBacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)DepositorInsertSNX5-PX (26-170) (SNX5 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYY1704
Plasmid#198421PurposepBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.DepositorInsertRedNTF
UseTagsExpressionPlantMutationPromoterpBs3Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP*-PTEN
Plasmid#86781PurposeFusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.DepositorInsertPTEN (PTEN Human)
UseTagsFKBP* (F36V and L106P) and linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P-1 SARS-CoV 3CLpro
Plasmid#61692Purposeinducible expression of SARS 3CLpro proteaseDepositorInsertSARS 3CLpro protease
UseTagsGST and HISExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YC3.60
Plasmid#48339PurposeFRET reporter for Ca2+ inside primary ciliaDepositorInsert5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
UseTagsYC3.60ExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Caveolin-3
Plasmid#68396PurposeFluorescently tagged Caveolin-3 for expression in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorInsertG4G6 (GSN Human)
UseTags6xHis-MBPExpressionBacterialMutationWTPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 CoV2
Plasmid#175512PurposeFor protein expression of WT nsp1 CoV2DepositorInsertnsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSMARCA4 guide 2
Plasmid#193605PurposeSMARCA4 knockoutDepositorInsertsgSMARCA4 guide 2 (SMARCA4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only