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pET-scFv-T

Plasmid

#67843

Purpose

pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Depositor

Joanna Bereta

Article

Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

Insert

antibody 1D8 light kappa chain

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pN565

Plasmid

#49990

Purpose

expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

T7 RNAP*

Use

Synthetic Biology

Tags

umuD degradation tag

Expression

Bacterial

Mutation

R632S

Promoter

pTac-symmetric

Availability

Academic Institutions and Nonprofits only

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

*

Promoter

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCELF PAI-RBP v1

Plasmid

#45506

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v1 (SERBP1 Rat, Human)

Use

Tags

GST tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCI-neo:alpha4-eGFP

Plasmid

#45068

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

alpha4-eGFP (Chrna4 Mouse)

Use

Tags

Green Fluorescent Protien and HA Tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBabe WT Akt2

Plasmid

#14551

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt2 (AKT2 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLVE-FH-IN

Plasmid

#70071

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short

Plasmid

#72552

Purpose

expresses 3*FLAG tagged human NSD3-short

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

none

Promoter

LTR

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-mRFP-pcDNA3.1-

Plasmid

#52510

Purpose

expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-mRFP-pcDNA3.1-

Plasmid

#52516

Purpose

expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-BLIMP-1-pcDNA3.1-

Plasmid

#52518

Purpose

expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blimp1 (PRDM1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAC1-pCR8-dCas9VP48

Plasmid

#48214

Purpose

dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP48 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP48

Expression

Mutation

D10A;H840A

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(Q71L)-HA

Plasmid

#79428

Purpose

Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(T31N)-HA

Plasmid

#79429

Purpose

Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-HseIF4E

Plasmid

#79437

Purpose

Expresses EGFP-tagged HseIF4E in Mammalian cells

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

HseIF4E (EIF4E Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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