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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v1

Plasmid

#97085

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAS

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Use

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6638

Plasmid

#106165

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-NED

Plasmid

#109369

Purpose

Lentiviral vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-E1347A-nCas9(D10A)-UGI

Plasmid

#187410

Purpose

DddA E1347A variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA E1347A variant

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_miniABEmaxNG(GG)

Plasmid

#187062

Purpose

miniABEmax(GG)-SpCas9-NG

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax(GG)-SpCas9-NG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAIO-LbCpf1

Plasmid

#137847

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAT15489_dABE8e

Plasmid

#174127

Purpose

plasmid expressing ABE8e with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a D832A

Plasmid

#113517

Purpose

Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

D832A

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AAAAA-nCas9(D10A)-UGI

Plasmid

#187409

Purpose

DddA AAAAA variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA AAAAA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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px459 VRER

Plasmid

#101716

Purpose

sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Use

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E and T1337R)

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAT15482_ABE8e

Plasmid

#174120

Purpose

Expresses ABE8e with an EF1a promoter

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eABE

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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