We narrowed to 59,970 results for: %s

1. pv6_MBD1_MBD

   Plasmid

   #179404

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv6_EGFP

   Plasmid

   #179392

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   EGFP
   

   Tags

   AviTag

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PIK3CA-WT

   Plasmid

   #16643

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA (PIK3CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH-SFB-BRCA1

   Plasmid

   #99394

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

   Depositor

   Junjie Chen, Michael Huen

   Article

   Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH-SFB-BRCA2

   Plasmid

   #99395

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

   Depositor

   Junjie Chen, Michael Huen

   Article

   Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   BRCA2 (BRCA2 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. fwYellow chromoprotein

   Plasmid

   #117841

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, fwYellow
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. scOrange chromoprotein

   Plasmid

   #117840

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, scOrange
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-NpHR-EYFP

    Plasmid

    #171621

    Purpose

    TRE-NpHR-EYFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-NpHR-EYFP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEVOL_PylRS(AF)-tRNA

    Plasmid

    #223511

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y306A; Y384F

    Promoter

    araBAD, rrnB, and glnS and proK

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV GFP

    Plasmid

    #49055

    Purpose

    adeno-associated virus (AAV) encoding a green fluorescent protein

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-DEST53 PINK1 N-GFP

    Plasmid

    #13315

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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