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qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al Nat Commun. 2025 Nov 13;16(1):9972. doi:

Insert

qMaLioffG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-Switch

Plasmid

#99733

Purpose

Flp-based FLEX reporter (from RFP to GFP)

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

TagRFP and EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95L_mCherry

Plasmid

#84022

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95L

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCaggs-mGold2s

Plasmid

#231763

Purpose

Expression of mGold2s in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Mammalian

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-vDIO-mScarlet-IRES-tTA

Plasmid

#118030

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-vDIO-mScarlet-IRES-tTA

Use

AAV

Promoter

TRE

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCas1_Cas2/3

Plasmid

#89240

Purpose

Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas1 and Cas2/3

Tags

Strep II tag on Cas1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB550

Plasmid

#74880

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pMito-Electra2

Plasmid

#184927

Purpose

Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

Glucagon-ICAM1-pre-mGRASP

Tags

3x HA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMyc0ElbLuc

Plasmid

#53242

Purpose

reporter plasmid without myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMycOElbLuc (MYC Human)

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKeima-Kan

Plasmid

#44902

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Expression

Yeast

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-10u

Plasmid

#13542

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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