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Plasmid#175952PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationZeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits
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MS2_SC2_S_Gamma
Plasmid#175047PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationGamma (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGRE2Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW295
Plasmid#139492PurposeInsertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpALD3 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpALD3Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP266
Plasmid#139488PurposeInsertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpCTT1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpCTT1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP264
Plasmid#139487PurposeInsertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW200
Plasmid#139489PurposeInsertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGPD1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGPD1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW293
Plasmid#139490PurposeInsertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpHSP12 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpHSP12Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4137385
Plasmid#149542PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
UseTagsExpressionMammalianMutationwt;18-aa CtruncPromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046565
Plasmid#149097PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSP64-GFP-ePDZ-CAAX
Plasmid#135603PurposeExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ-CAAX
UseIn vitro transcription (mrna synthesis)TagsCAAX, EGFP, and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-EMTB-GFP-ePDZ
Plasmid#135600PurposeExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertEMTB-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-membrane-mCherry-LOV
Plasmid#135599PurposeExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertmembrane-mCherry-LOV
UseIn vitro transcription (mrna synthesis)TagsLOV and mCherryExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP-ePDZ
Plasmid#135602PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pstb-LAFR5-exoY
Plasmid#86170Purposepstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/BamDepositorInsertexoY
UseTagsExpressionBacterialMutationPromoterSmb21651Available sinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW8ENdeI-cyEGFP-6XDSR
Plasmid#49865Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-3XDSR
Plasmid#49863Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-6XDSR
Plasmid#49870Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only