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pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

Plasmid

#166873

Purpose

AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-VEGFA array presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EFS and U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #1

Plasmid

#27016

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #1 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pRR-GR-5Z

Plasmid

#23060

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

GR (NR3C1 Human)

Expression

Yeast

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-Kras

Plasmid

#140838

Purpose

mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-Kras

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-N-CretrcintG

Plasmid

#69572

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - N-CretrcintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-C-CreintG

Plasmid

#69573

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - C-CreintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-PTC39

Plasmid

#130701

Purpose

Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Expression

Mammalian

Mutation

PTC39

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Worm and Yeast

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry

Plasmid

#192634

Purpose

Lentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-KRasCT

Plasmid

#175835

Purpose

Express mCherry with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-NaChBac

Plasmid

#125580

Purpose

Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

NaChBac

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S277G

Plasmid

#13615

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic Serine 277 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
3×Cherry Dam AID Smash

Plasmid

#227374

Purpose

For inducible expression and cytosolic localization of Dam

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

Dam DNA-adenine methyltransferase

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CSIV-TRE-ASCL1

Plasmid

#154883

Purpose

Doxycycline-dependently expresses human ASCL1.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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