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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC26A7

Plasmid

#132152

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A7 (SLC26A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti_EF1a_Flag_TP53_P278A

Plasmid

#183113

Purpose

Expresses flag-tagged p53 with P278A mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine, see depositor com…

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Expression

Mutation

Promoter

CamK II

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_WT

Plasmid

#164936

Purpose

Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Expression

Mutation

Promoter

PGK promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K120R/K164R)

Plasmid

#72556

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

K120R/K164R

Promoter

E1B minimal promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC26A11

Plasmid

#131996

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A11 (SLC26A11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

Plasmid

#134922

Purpose

Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STINGHAQ

Plasmid

#102587

Purpose

Lentivector to express STING HAQ and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_UCP3

Plasmid

#132258

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP3 (UCP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-puro-tetON-shOGDH 2497

Plasmid

#110942

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC30A3

Plasmid

#132101

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A3 (SLC30A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

Plasmid

#134924

Purpose

Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LentiRCas9-Lambda2

Plasmid

#104184

Purpose

Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Article

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-sgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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WT-β3

Plasmid

#166579

Purpose

For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

β3 (Itgb3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL G44S (siRNA resistant)

Plasmid

#191012

Purpose

Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

G44S

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shOGDH 1714

Plasmid

#110941

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.1

Plasmid

#208020

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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