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pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Publication

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
plRL118

Plasmid

#163632

Purpose

Renilla luciferase reporter plasmid; Giles integrating.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

Luciferase

Expression

Bacterial

Promoter

Mycobacterial Optimized Promoter (MOP)

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1

Plasmid

#135503

Purpose

Mammalian expression of myc-tagged HLA-A*01:01

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR1a-mCherry-STIM1

Plasmid

#114176

Purpose

Gateway entry clone containing mCherry-STIM1

Depositor

Nicolas Demaurex

Publication

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Gateway Cloning

Tags

mCherry (inserted after signal peptide)

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Td-CGBE

Plasmid

#196599

Purpose

Mammalian expression of Td-CGBE

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N46L); S. pyogenes Cas9 (D10A)

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7 K64M

Plasmid

#75143

Purpose

Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lys 64 mutated to Met; kinase inactive and F236L …

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Publication

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-KLF4

Plasmid

#35389

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Klf4

Use

Lentiviral

Expression

Mammalian

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pENTR11 mSTIM2 myc

Plasmid

#17734

Depositor

Anjana Rao

Publication

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM2 (Stim2 Mouse)

Tags

Myc

Expression

Bacterial

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pNIR-FbGFP

Plasmid

#184675

Purpose

NIR-Fb to GFP

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbGFP

Expression

Mammalian

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Publication

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

Plasmid

#165456

Purpose

Inducible ZEB2 expression from AAVS1 locus

Depositor

Madeline Lancaster

Publication

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

ZEB2 (ZEB2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Publication

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin-S3D

Plasmid

#78297

Purpose

Mammalian expression of mouse cofilin S3D fused to EGFP

Depositor

Michael Frotscher

Publication

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

S3D

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

Plasmid

#230803

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11528 - pAAV-hsA2-eHGT_082h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1528)

Plasmid

#163502

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

MinRho

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCW-eGFP-FHA-SHLD2

Plasmid

#114121

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH-EF1a-extGluc-mKate-NGFR

Plasmid

#104831

Purpose

extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

Depositor

Irmela Jeremias

Publication

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

mKate
NGFR

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-PSDΔ1,2-FRBx3

Plasmid

#225827

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Publication

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

PSDΔ1,2-FRBx3

Use

AAV

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PE

Plasmid

#24144

Depositor

Ines Ibanez-Tallon

Publication

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Expression

Mammalian

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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