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pMW364

Plasmid

#238205

Purpose

Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-MEKK1-1-1493

Plasmid

#21632

Depositor

Melanie Cobb

Article

Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

Insert

mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCW57.1 WT1 +1 ATG

Plasmid

#217643

Purpose

Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 long isoform (WT1 Human)

Expression

Mammalian

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 243-450

Plasmid

#24392

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 243-450.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1 151-241

Plasmid

#24390

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 151-241.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12g1

Plasmid

#120879

Purpose

Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12g1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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SB_loop

Plasmid

#110103

Purpose

pBXPHM3 with scaffold Sybody of the loop library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the loop library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAV116

Plasmid

#63183

Purpose

pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cluc-RMA

Plasmid

#189624

Purpose

Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

Cypridina luciferase fused to Fc

Use

AAV and Luciferase

Tags

Cluc and IgG1 Fc

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

Plasmid

#153527

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#68

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#68

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_SA-2x

Plasmid

#79369

Purpose

Assays activity of transcriptional activators fused to SA Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Circular-ic-CVB3-GFP

Plasmid

#226260

Purpose

Production of in situ circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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