We narrowed to 59,100 results for: %s

1. pAV10.K5

   Plasmid

   #63210

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-HE-Venus-bPAC(F198Y)

   Plasmid

   #165487

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Venus-bPAC(F198Y)-myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-mGold-Lysosome-N-20

   Plasmid

   #158006

   Purpose

   Visualization of lysosome in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMGIB-HA-hTCAB1

   Plasmid

   #167460

   Purpose

   Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9 TadCBEb

   Plasmid

   #193832

   Purpose

   Express TadCBEb (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDb-SpCas9 D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN_HA-TBK1 (E696K)

   Plasmid

   #199207

   Purpose

   Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   E696K

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneoLuc-PP1A

   Plasmid

   #128348

   Purpose

   Vector for LUMIER assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   PP1A (PPP1CA Human)
   

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

    Plasmid

    #160642

    Purpose

    Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    GRLacIBDGal4AD / RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-H2-Ld

    Plasmid

    #135519

    Purpose

    Mammalian expression of myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. dmBACCS2VL-IRES-dOrai-IRES-mCherry

    Plasmid

    #72898

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-mCherry
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PIK3CA - Exon20 H1047R (pFastBac)

    Plasmid

    #16641

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.shNkx2-1

    Plasmid

    #32400

    Depositor

    Tyler Jacks, Monte Winslow

    Article

    Winslow et al Nature. 2011 May 5. 473(7345):101-4.

    Insert

    shRNA targetting Nkx2-1 (Nkx2-1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

    Plasmid

    #13617

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 65, Thr 69 and Ser70 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pTol2CG2-QUASM1:mScarletNLS-SV40pA

    Plasmid

    #155124

    Purpose

    Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:mScarletNLS
    

    Use

    Zebrafish expression

    Promoter

    QUASM1-E1b

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-p97-AE-mycStrep

    Plasmid

    #31841

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    A232E

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

18. lentiCRISPR.sgKras.9

    Plasmid

    #91894

    Purpose

    sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

    Depositor

    Wen Xue

    Article

    Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Kras sgRNA
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-TFAP4(E135A/S139A)

    Plasmid

    #110157

    Purpose

    Expression of human TFAP4 (E135A/S139A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E135A/S139A

    Promoter

    CMV

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. dmBACCS2NS-IRES-dOrai-IRES-mCherry

    Plasmid

    #72896

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-mCherry
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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