We narrowed to 59,952 results for: %s

1. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Lambda-PPase
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-Gluc-RMA-IRES-EGFP

   Plasmid

   #189629

   Purpose

   Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Promoter

   IRES and hSyn

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

   Plasmid

   #153005

   Purpose

   Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (C) (G79)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV GFP

   Plasmid

   #49055

   Purpose

   adeno-associated virus (AAV) encoding a green fluorescent protein

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCutamp

   Plasmid

   #140632

   Purpose

   Plasmid-curing in Escherichia coli by targeting the AmpR promoter

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-DEST53 PINK1 N-GFP

   Plasmid

   #13315

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS3Clux

    Plasmid

    #55172

    Purpose

    lux-reporter vector, integration at sacA, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-cIAP2/pRK5

    Plasmid

    #27973

    Depositor

    Xiaolu Yang

    Article

    Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

    Insert

    CIAP2 (BIRC3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS2E

    Plasmid

    #55169

    Purpose

    Empty vector, integration at lacA, ampr, mlsr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Tags

    myc, His

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDXinit

    Plasmid

    #110101

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. SpCas9 TadCBEd

    Plasmid

    #193835

    Purpose

    Express TadCBEd (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS2002

    Plasmid

    #239832

    Purpose

    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB omegaRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Replacement of A12-A57 with GAAA in omegaRNA scaf…

    Promoter

    human U6

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pClneoMyc human CIN85

    Plasmid

    #47935

    Purpose

    expresses Myc tagged human CIN85 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Kawata et al J Biochem. 2006 May;139(5):931-9.

    Insert

    CIN85 (SH3KBP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    contains amino acids 2-665 of human CIN85; D114G

    Promoter

    CMV

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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