We narrowed to 59,462 results for: %s

1. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120298

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA5/mCherry-pBirA-HA

   Plasmid

   #154458

   Purpose

   expresses mCherry-pBirA-HA/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry-pBirA-HA
   

   Expression

   Mammalian

   Promoter

   CMV-F, T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS1929

   Plasmid

   #205968

   Purpose

   JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH crRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-hPPP3R1

   Plasmid

   #179136

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TIA1 RRM-YFP-SspBx6

   Plasmid

   #103781

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-YFP-SspBx6
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-bArr1

   Plasmid

   #201491

   Purpose

   Expression of the beta-arrestin1 containing a N-terminal V5 tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   V5-bArr1 (ARRB1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp128-CUP1-NUbo-GFP-His6

   Plasmid

   #212790

   Purpose

   Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-GFP-His6
   

   Use

   Integrative vector

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-ubiquitin-SV40-RFP

    Plasmid

    #65448

    Purpose

    Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-RFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBQ

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GST-PRDM16 880-1038

    Plasmid

    #53350

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 880-1038

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. GST-PRDM16 224-454

    Plasmid

    #53347

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 224-454

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. attB-­GFP-­Poly(A)‐NeoR

    Plasmid

    #65526

    Purpose

    NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS1477

    Plasmid

    #205970

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH all proteins, with His-tagged Cas11
    

    Tags

    14xHis on Cas11

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LbCas12a-DR-cr in PBCSK-

    Plasmid

    #126641

    Purpose

    Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-PRDM16 1-223

    Plasmid

    #53346

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-223

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGEL636

    Plasmid

    #189830

    Purpose

    The T-DNA backbone of Cas12j2 for transcriptional activation in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    pZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Promoter

    ZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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