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Plasmid#138459Purposelenti vector encoding the MS2-p300 with a 2A Hygro resistance markerDepositorInsertp300 core
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi
Plasmid#85455PurposemWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341APromoterTlpA and TlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQF-mNGsacB
Plasmid#203673PurposepQF with mNeonGreen and sacB under control of PQ5DepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQ5Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
bactin-loxp-BFP-loxp-dsred-pBSII
Plasmid#68972Purposefor transgenic animalsDepositorInsertslox-BFP-loxp
dsred
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RNF169
Plasmid#119004PurposeMammalian expression of human RNF169 and BFPDepositorInsertRNF169 (RNF169 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS402-nicastrin/APH-1a-HA
Plasmid#207804PurposeYeast integrative vector for expression of Nicastrin and APH-1a-HADepositorInsertGamma-secretase subunits nicastrin and Aph1a
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-SpHis5
Plasmid#44841PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selectionDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
prCAG-BoxB
Plasmid#191122PurposeTranscription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
BoxB
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM733
Plasmid#68898PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-short
Plasmid#51546Purposep2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
1455_pDEST_miniTol2_R4-R2_MCS
Plasmid#171794PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-CaURA3
Plasmid#44872PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer P-null replicon
Plasmid#65462Purposecontaining 1.3 units of the P-null HBV genome (subtype ayw)DepositorInsert1.3 units of the P-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC0090
Plasmid#181954PurposeBeta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/ClucDepositorInsert7xTOPFlash
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-GCaMP6s
Plasmid#184712PurposeCalcium biosensorDepositorInsertGCaMP6s
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only