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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_PYGL_WT_V5

Plasmid

#82991

Purpose

Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PYGL (PYGL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku80

Plasmid

#46958

Purpose

Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku80 (XRCC5 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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NLS-LucWT-GFP

Plasmid

#127709

Purpose

The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

Luciferase

Use

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0 FLAG TRPV4

Plasmid

#45751

Depositor

Robert Lefkowitz

Article

Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

Insert

TRPV4 (Trpv4 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pTracer-HV1

Plasmid

#24710

Depositor

Yuriy Kirichok

Article

Lishko et al Cell. 2010 Feb 5. 140(3):327-37.

Insert

HV1 (HVCN1 Human)

Use

Tags

GFP--NOT a fusion!

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF1062

Plasmid

#143751

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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