-
Plasmid#129109PurposeExpresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoterDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
GGE142
Plasmid#120735PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)DepositorInsertMarker_Suc2
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-FRB-LplA(W37V)
Plasmid#35608DepositorInsertFRB-LplA(W37V)
UseTagsFLAG (internal fusion), HA, and His TagExpressionMammalianMutationLplA has Trp37 to Val mutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
rNrx1beta(47-299)
Plasmid#20173DepositorInsertNeurexin 1 beta (Nrxn1 Rat)
UseTags6X His tagExpressionInsectMutationno splice sitePromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosTagsExpressionMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2ALexA
Plasmid#125220PurposeArtificial phase 0 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertLexA
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-iRFP702-NES
Plasmid#184457PurposeCytosolic iRFP702 expression in mammalian cellsDepositorInsertiRFP702-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC150
Plasmid#70014Purpose'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral RepliconDepositorInsertDonor + GVR
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mouse NCKX4-AS-long (pcDNA3.1+)
Plasmid#75208PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (Slc24a4 Mouse)
UseTagsExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c011)
Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]
Plasmid#159797PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Pmlc-2 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet11a-ABLE
Plasmid#158627PurposeAmp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expressionDepositorInsertApixaban-Binding Helical Bundle
UseTags6x His-tag and TEV cleavage tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMPO 61
Plasmid#69869PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, MCS M13 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPO 58
Plasmid#68362PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
rNL1(46-638)
Plasmid#20172DepositorInsertNeuroligin 1 (Nlgn1 Rat)
UseTagsC-terminal 6X His tagExpressionInsectMutationno splice sitesPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
YCe1891 HC_Kan_SV40-ORI_p25
Plasmid#100679Purposepart designed to occupy position 25 of EMMA. Functional category: Viral origin of replicationDepositorInsertSV40-ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2AGeneSwitch
Plasmid#125214PurposeArtificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_160
Plasmid#91206PurposeTransformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only