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Plasmid#100150PurposeExpresses EME1DepositorInsertEME1 (EME1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PresentER-NLVPMVATV (GFP)
Plasmid#102947PurposePresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)DepositorInsertNLVPMVATV
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-pcDNA3.1
Plasmid#85760Purposemammalian expression of full length HA tagged USPL1DepositorInsertUSPL1, full length (USPL1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mSNX13-PX (558-677)
Plasmid#119094PurposeBacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)DepositorInsertmSNX13-PX (558-677)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP
Plasmid#199367PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOEu-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mCherry-WPRE
Plasmid#202620PurposeDouble floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertmCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-p38beta
Plasmid#86833Purposemammalian expression of GFP-p38betaDepositorInsertp38beta (MAPK11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV gfaABC1D NES-jRCaMP1a
Plasmid#171120PurposeAstrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.DepositorInsertjRCaMP1a
UseAAVTagsHis Tag, T7 Tag, XpressTagExpressionMammalianMutationPromotergfaABC1DAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
hRAD18-EGFP
Plasmid#68824PurposeExpresses human RAD18 tagged with EGFPDepositorInserthuman RAD18 tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)
Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
UseTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATG9A-2STrep
Plasmid#210359PurposeExpresses ATG9A fused with 2Strep in mammalian cellsDepositorInsertATG9A (ATG9A Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf170
Plasmid#12858PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf170
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1-mut
Plasmid#188570Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-HSPB8
Plasmid#34710DepositorInsertheat shock 22 kDa protein 8 (HSPB8 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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A&C-BEmax
Plasmid#157947PurposeMammalian expression of A&C-BEmaxDepositorInsertA&C-BEmax
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only