Addgene

We narrowed to 27,570 results for: eng

Showing: 10441 - 10460 of 27570 results

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pc3XB-ZF94

Plasmid

#13228

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF94

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF97

Plasmid

#13231

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF97

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF99

Plasmid

#13233

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF99

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF101

Plasmid

#13235

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF101

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF104

Plasmid

#13238

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF104

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF105

Plasmid

#13239

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF105

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Use

Tags

Expression

Insect

Mutation

K986N- see depositor comments below

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper delta ITAM

Plasmid

#126671

Purpose

Expression of His10-Draper delta ITAM mutant for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic ITAM residues mutated to Phe

Use

Tags

Expression

Bacterial

Mutation

Cytoplasmic ITAM residues mutated to Phe

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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C077

Plasmid

#174346

Purpose

Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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C078

Plasmid

#174347

Purpose

Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

13 silent mutations: G243A, A273G, G339A, C345G, …

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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C233

Plasmid

#174349

Purpose

Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (M161A) (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

M161A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Article

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRASV12

Plasmid

#20205

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

NRas (NRAS Human)

Use

Transposon

Tags

Expression

Mutation

G12V

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2

Plasmid

#99868

Purpose

Measure FRET between LSSmOrange and mKate2

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

LSSmOrange

Use

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_ATP1.0

Plasmid

#167581

Purpose

Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-pre-eGRASP(p32)

Plasmid

#111598

Purpose

An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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