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pFL-EGFP-3xFlag-Fml1

Plasmid

#193058

Purpose

Baculovirus expression of S.pombe Fml1 for recombinant protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Fml1 (fml1 Fission Yeast)

Use

Tags

3xFlag

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-CDDR

Plasmid

#149355

Purpose

CRISPR/Cas9-based dual-fluorescent DSB repair reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

CDDR Reporter
Blasticidin resistance

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a and hPGK

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199366

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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FR_HNF1A

Plasmid

#31104

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

HNF1A (HNF1A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Pclo KI

Plasmid

#139657

Purpose

Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1E10A

Plasmid

#84607

Purpose

Plasmid contains HCF-1rep1E10A with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1E10A (HCFC1 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1wt

Plasmid

#84606

Purpose

Plasmid contains HCF-1rep1wt with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1wt (HCFC1 Human)

Use

Tags

GST-TEV and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-CD82

Plasmid

#1819

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCI-ING1a

Plasmid

#79069

Purpose

Expression of p47ING1 in mammalian cells

Depositor

Karl Riabowol

Article

Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

Insert

ING1 (p47 ING1A isoform) (ING1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDC-UAS-nBitbow1.0(miniW)

Plasmid

#138329

Purpose

Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

Depositor

Dawen Cai

Article

Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

Inserts

mAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2

Use

Tags

human H2B

Expression

Insect

Mutation

Promoter

10xUAS

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-UbxD8

Plasmid

#53777

Purpose

express FLAG-tagged human UbxD8 gene

Depositor

Yihong Ye

Article

Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

Insert

UbxD8 (FAF2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry 14-3-3β ctrl

Plasmid

#112031

Purpose

Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Insert

14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry

Use

Tags

Split mCherry

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn3

Plasmid

#164959

Purpose

Zebrafish kcnn3 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn3 (kcnn3 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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