-
Plasmid#204555PurposeExpresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cellsDepositorInsertCDK5RAP3 (AMY2A Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK1-K179E
Plasmid#199644PurposeExpresses kinase-inactive VRK1 (K179E)DepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsExpressionMutationK179EPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Dcst1-3xFLAG
Plasmid#183542PurposeExpression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.DepositorInsertDC-STAMP domain containing 1 (Dcst1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-R1441C
Plasmid#25046DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A45
Plasmid#132054PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A45 (SLC25A45 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-Dcst2-3xHA
Plasmid#183543PurposeExpression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.DepositorInsertDC-STAMP domain containing 2 (Dcst2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ai32
Plasmid#34880PurposeCre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitationDepositorInsertFloxed ChR2(H134R)-EYFP
UseMouse TargetingTagsEYFPExpressionMutationH134R in ChR2PromoterCAGAvailable sinceMay 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNAâ„¢5/FRT/CMV_promoter-Cas9-E2A-mRFP
Plasmid#164131PurposeExpresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)DepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0med
Plasmid#153292PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
Plasmid#118594PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertsgRNA/Puro-T2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-SOK
Plasmid#20327DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-BiPOLES-mCerulean
Plasmid#154950PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-Flag-SREBP1
Plasmid#134283PurposeExpresses Flag-tagged SREBP1 in mammalian cellsDepositorInsertSREBP1 (Srebf1 Mouse)
UseTagsFlagExpressionMammalianMutationmouse SREBP1 isoform a precursorPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-mFoxP1
Plasmid#35170DepositorInsertFoxp1 (Foxp1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M
Plasmid#102591PurposeLentivector to express STING V155M and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155MPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tead4
Plasmid#128832Purposedox-dependent expression of mouse Tead4DepositorInsertTead4 (Tead4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertCRISPR Cas9
UseCRISPRTagsExpressionMammalianMutationCodon usage optimized for human usagePromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only