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Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDG_SCN3B_OE_1
Plasmid#161625PurposeOverexpresses SCN3BDepositorInsertSCN3B (SCN3B Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab8A
Plasmid#166428PurposeRab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab8A
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Nesprin-3
Plasmid#175136PurposeLentiviral expression of V5-tagged mouse Syne3DepositorInsertSyne3 (Syne3 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tubulin-6
Plasmid#62736PurposeLocalization: Microtubules, Excitation:488, Emission:507DepositorInsertTubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS L30 mRuby3-Rab10
Plasmid#166429PurposeRab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab10
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSV40-GUS-P2A-Hygro
Plasmid#106480PurposeExpression of GUS and Hygromycin resistance gene from SV40 promoterDepositorInsertsbeta-glucuronidase
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIC79
Plasmid#63063PurposeMammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)DepositorInserthNSL1, IMAGE clone 3997280 (NSL1 Human)
UseTagsEYFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pIVTR(T7A)-Cas9n-RT-T2A-PuroR
Plasmid#172296PurposeIn vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editingDepositorInsertCas9n-RT-T2A-PuroR
UseCRISPRTagsExpressionMutationH840A (Cas9)PromoterT7 promoter A-initiatingAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his24::tagBFP2
Plasmid#124344PurposeNuclear Localized Pan-Neuronal tagBFP2DepositorInsertrab3, tagBFP2 (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Clover2-C1
Plasmid#54711PurposeLocalization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.DepositorTypeEmpty backboneUseTagsClover2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG497 mito-iFAST
Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBS L30 mRuby3-Rab11A
Plasmid#166430PurposeRab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab11A
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE502B
Plasmid#128971PurposeBroad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxyl-PmAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE
Plasmid#186047PurposeAAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expressionDepositorInsertLifeAct-jGCaMP8f
UseAAVTagsLifeAct-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only