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Tol2-lyzC-Vector-Dendra2

Plasmid

#97101

Purpose

empty vector for overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpRY-P2A-GFP

Plasmid

#159981

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpRY-P2A-GFP

Use

Tags

BpSV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H3.3

Plasmid

#42632

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Use

Tags

Untagged

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-hPR-A

Plasmid

#89119

Purpose

mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

human progesterone receptor-A, equivalent to huma…

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET 28 full-length human PARP1

Plasmid

#192635

Purpose

Expresses human PARP1 with an N-terminal His-tag

Depositor

John Pascal

Article

Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

Insert

Human PARP1 (PARP1 Human)

Use

Tags

histidine-tag

Expression

Bacterial

Mutation

Polymorphism A762

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p28-2iDMD-puro

Plasmid

#88895

Purpose

28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

Depositor

Michele Calos

Article

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dystrophin (DMD Human)
luciferase
puromycin resistance enzyme
mCherry

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Cavb3

Plasmid

#26574

Depositor

Diane Lipscombe

Insert

calcium channel beta subunit-III (Cacnb3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTdTomato-L5

Plasmid

#140994

Purpose

tdTomato, StrepR, L5int, attP to create RFP mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

hsp60

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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