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pFIN-EF1a-CHER-WPRE

Plasmid

#44353

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

EF1a-CHER

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human elongation factor -1a (pos 2526-3969)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-oLID-CAAX (from KRas4B)

Plasmid

#60412

Purpose

Mammalian Expression of Venus-oLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-oLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERGIC1__RANBP17

Plasmid

#116816

Purpose

Lentiviral expression of ERGIC1__RANBP17 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERGIC1__RANBP17

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDest-665 (attR4-attR2)

Plasmid

#161885

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148852)

Plasmid

#80175

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147812)

Plasmid

#80176

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-MCM5__LYN

Plasmid

#116828

Purpose

Lentiviral expression of MCM5__LYN fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MCM5__LYN

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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INSR gRNA (BRDN0001145804)

Plasmid

#75506

Purpose

3rd generation lentiviral gRNA plasmid targeting human INSR

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

INSR (INSR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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MAP4K2 gRNA (BRDN0001144728)

Plasmid

#76779

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K2 gRNA (BRDN0001146560)

Plasmid

#76780

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK6-P2A-Hygro_Barcode

Plasmid

#170224

Purpose

Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001149237)

Plasmid

#77833

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TWF1 gRNA (BRDN0001149022)

Plasmid

#77287

Purpose

3rd generation lentiviral gRNA plasmid targeting human TWF1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TWF1 (TWF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TWF1 gRNA (BRDN0001147784)

Plasmid

#77288

Purpose

3rd generation lentiviral gRNA plasmid targeting human TWF1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TWF1 (TWF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Tuba RNAi

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
TTVTFLPTPSLILQT_epitope

Plasmid

#165021

Purpose

Expression of CMV UL44 (38-52) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (38-52)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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