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pDSP31
Plasmid#199373PurposeEncodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP23
Plasmid#199372PurposeEncodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam8_N_Flag_3C
Plasmid#178818PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, 3CExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGHE-PdNIP2-1
Plasmid#108447PurposePdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.DepositorInsertPdNIP2-1
UseTagsExpressionMutationPromoterpT7Available sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NES-jGCaMP8f-WPRE
Plasmid#186044PurposeAAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expressionDepositorInsertNES-jGCaMP8f
UseAAVTagsNES-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-sfGFP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-Blast
Plasmid#158557PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam7_N_Flag_TEV
Plasmid#178817PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NES-jGCaMP8m-WPRE
Plasmid#186045PurposeAAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expressionDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJR81
Plasmid#75029PurposetagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInserttagBFP2^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized tagBFP2ExpressionWormMutationPromoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pNSU299_SEO1
Plasmid#166076PurposePlasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertSEO1 (SEO1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
YARS_pNIC-CTB10H
Plasmid#153059PurposeExpression construct for producing Avi tagged Tyrosyl-tRNA synthetaseDepositorInsertYARS (YARS1 Human)
UseTagsTEV-Avi-His10ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pnos-Fok1:dCas9-nos
Plasmid#62210PurposeExpresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
UseTagsExpressionInsectMutationPromoternanosAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLIX403-EBFP2-ccdB-NatMx
Plasmid#158559PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(0)
Plasmid#163426PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Sun2
Plasmid#175114PurposeLentiviral expression of V5-tagged mouse Sun2DepositorInsertSun2 (Sun2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only
