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Plasmid#199308PurposeMosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertmyo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promotermyo-3Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hPhyBY276H-mCherry-HRasCT
Plasmid#100540PurposeExpresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
UseTagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-908 aa), Y276H muta…PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC5A2
Plasmid#132241PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC5A2 (SLC5A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC38A7
Plasmid#132094PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC38A7 (SLC38A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HLA-A0201-His
Plasmid#108213PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseEntry vector for gateway systemTagshisExpressionMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
Plasmid#154881PurposeDoxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ Myc2-mouse TTP WT
Plasmid#107000PurposeExpresses epitope-tagged wild-type murine TTPDepositorInserttristetraprolin (Zfp36 Mouse)
UseTagsDouble Myc peptide epitope tagExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A3
Plasmid#132114PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A3 (SLC2A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TetO-FUW-Foxo1
Plasmid#118588PurposeExpresses Foxo1 from the TRE3G promoterDepositorInsertFoxo1 (Foxo1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pED9x (dCas9-KRAB-mCherry)
Plasmid#163956PurposeLentiviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
UseTagsExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Venus
Plasmid#70267PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Venus Reporter
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NS and P2AAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-diLACCO1
Plasmid#208102PurposeControl biosensor for intracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-UFSP2
Plasmid#204553PurposeExpresses 3xFLAG-tagged UFSP2 in mammalian cellsDepositorInsertUFSP2 (UFSP2 Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC9A7
Plasmid#132187PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9A7 (SLC9A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits