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Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMU-NUCr
Plasmid#61168PurposeFluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoterDepositorInsertNLS-mCherry-GUS
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMU-ACTFr
Plasmid#61191PurposeFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoterDepositorInsertAtFimbrin1-ABD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hs.NRAS G12V
Plasmid#83174PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutationDepositorInsertneuroblastoma RAS viral oncogene homolog (NRAS Human)
UseGateway entry cloneTagsExpressionMutationG12VPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Ubi
Plasmid#99540PurposeYeast episomal vector for expression of His-tagged ubiquitin; URA3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-FAK-HA
Plasmid#35039DepositorInsertfocal adhesion kinase (Ptk2 Mouse)
UseTags2x HA and mCherryExpressionMammalianMutationY42H; deletion of amino acids 1051 & 1052PromoterAvailable sinceMarch 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAT75
Plasmid#204306PurposeFlippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthpartsDepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_G78R
Plasmid#113047PurposeExpression of mutant hSDH5DepositorInserthSDH5_G78R (SDHAF2 Human)
UseTagsExpressionBacterialMutationG78R, Insert begins at amino acid 39PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_C83D
Plasmid#113048PurposeExpression of mutant hSDH5DepositorInserthSDH5_C83D (SDHAF2 Human)
UseTagsExpressionBacterialMutationC83D, Insert begins at amino acid 39PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Ubi
Plasmid#99539PurposeYeast episomal vector for expression of His-tagged ubiquitin; LEU2 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB701NL3F10H
Plasmid#141288PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only