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Plasmid#166133PurposeThis plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting cldn1 gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro
Plasmid#166134PurposeThis plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting firefly luciferase gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-K206A)-IRES-puro
Plasmid#128679Purposemammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/K206APromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-KTM)-IRES-puro
Plasmid#128676Purposemammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/-K41R/K61R/K81RPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-K244A)-IRES-puro
Plasmid#128680Purposemammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/K244APromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS1_Flag (Variant: W98G)
Plasmid#79641PurposeThird generation Self-inactivating lentivector expressing NS1 (Variant W98G) from Zika virusDepositorInsertNS1
UseLentiviralTagsFlagExpressionMammalianMutationVariant: W98GPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA pBabe-puro
Plasmid#120308PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorTypeEmpty backboneUseRetroviralTags-BioID2-HAExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX_403
Plasmid#41395PurposeInducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTADepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsV5ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-pur H-2Kb
Plasmid#111623PurposeSleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I alleleDepositorInsertH2Kb
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-EGFP-Hu_Plk1(WT)
Plasmid#136323PurposeRetroviral expression of human Plk1-WT with N-terminal EGFPDepositorInsertPLK1 (PLK1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLV-Puro_hCOL4A4
Plasmid#198099Purposelentiviral expression of hCOL4A4 in mammalian cellsDepositorInsertCOL4A4 (COL4A4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgATG7
Plasmid#211534PurposeDeletes ATG7DepositorInsertsgATG7
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ePEG-LINE1-loxPsym
Plasmid#216866PurposeLentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elementsDepositorInsertspacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL11
Plasmid#189609PurposeRetroviral expression vector for IL11DepositorInsertIL11 (IL11 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro cyclinD1 HA
Plasmid#9050DepositorInsertcyclin D1 (CCND1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_roGFP2_Orp1
Plasmid#155043PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-iRFP-ccdB-Puro
Plasmid#158566PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsiRFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-10X-CS1-3'-puro
Plasmid#212011PurposeDeliver gRNA through lentivirus (compatible with 10x capture Sequence 1)DepositorInsertgRNA scaffold
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
katushka-FMDV-P2A-pTRIP
Plasmid#163522PurposeUsed to construct lentivirus to express katushka in mammalian cells with option for a second insertDepositorInsertstopless Katushka-P2A
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only