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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Use

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-CCPB1-C-18

Plasmid

#54027

Purpose

Localization: Actin Capping Protein , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

CCPB1

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-CCPB1-N-18

Plasmid

#54028

Purpose

Localization: Actin Capping Protein , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

CCPB1

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEGFP-Lifeact-7

Plasmid

#54610

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mEGFP-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mTagBFP-Lifeact-7

Plasmid

#54496

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mTagBFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mApple-Lifeact-7

Plasmid

#54747

Purpose

Localization: Actin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mApple-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-VASP-5

Plasmid

#55151

Purpose

Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

VASP

Use

Tags

mCherry

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mRuby-Lifeact-7

Plasmid

#54560

Purpose

Localization: Actin, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

LifeAct- mRuby

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEmerald-Espin-like-C-18

Plasmid

#54086

Purpose

Localization: Actin Bundling , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Espin-like

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
EBFP2-Lifeact-7

Plasmid

#54559

Purpose

Localization: Actin, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

EBFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
sfGFP-Lifeact-7

Plasmid

#54739

Purpose

Localization: Actin, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mPlum-Lifeact-7

Plasmid

#54679

Purpose

Localization: Actin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mPlum-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
mKate-Lifeact-7

Plasmid

#54697

Purpose

Localization: Actin, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

LifeAct- mKate

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mCitrine-Lifeact-7

Plasmid

#54733

Purpose

Localization: Actin, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mCitrine-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-VASP-N-10

Plasmid

#54297

Purpose

Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

VASP

Use

Tags

mEmerald

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits