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Plasmid#205274PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD487KPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
Plasmid#205272PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD300RPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ273: pRS426-His-MBP-GtFz1_PSP1
Plasmid#205258PurposeS. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)DepositorInsertGtFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ127: pRS426-His-MBP-NlovFz2_PSP1
Plasmid#205257PurposeS. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)DepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH361 Hspa8 MS2 HA
Plasmid#168218PurposeMammalian expression of heat shock 70 kDa protein 8 fused to the MS2 dimer and C-terminal HA tagDepositorInsertHspa8
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c011)
Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pLITMUS-rpoN-cI5C6A-J23106-geneIII
Plasmid#80905PurposePhagemid (encodes cI5C6A and M13 gene III)DepositorInsertsM13 gene III
cI5C6A
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G,P-J23106-geneIII
Plasmid#80861PurposePhagemid (encodes evolved cI5G6G,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6G,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTNT-pT7-tetR-sfGFP
Plasmid#140868PurposeT7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)DepositorInsertTetR-sfGFP expressed with T7 promoter
UseSynthetic BiologyTagssfGFPExpressionBacterialMutationPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPELL-Lyn Component 1
Plasmid#170484PurposeSPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.DepositorInsertLyn(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-Lyn Component 2
Plasmid#170485PurposeSPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.DepositorInsertFRB, Lyn(C-lobe)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM23
Plasmid#74643PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII
Plasmid#80862PurposePhagemid (encodes evolved cI5G6T,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c104)
Plasmid#98234PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G-J23106-geneIII
Plasmid#80906PurposePhagemid (encodes cI5G6G and M13 gene III)DepositorInsertsM13 gene III
cI5G6G
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only