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Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK85
Plasmid#176611PurposeTo minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.DepositorInsertSNAP-FCERG-mGFP
UseLentiviralTagsExpressionMammalianMutationC25APromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK83
Plasmid#176610PurposeTo measure Syk intensity in response to ligand clustering using DNA origamiDepositorInsertNeonGreen-tSH2 Syk (Syk Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)
Plasmid#179080PurposeParent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminatorsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179026PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179025PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179024PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179023PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179022PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179021PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK36 pHomL-RPL18Bp-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179020PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK35 pHomL-TEF2p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179019PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK34 pHomL-TDH3p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179018PurposeParent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK33 pHomL-RPL18Bp-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179017PurposeParent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK32 pHomL-TEF2p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179016PurposeParent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179014PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179013PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179011PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-SpEdc1opt_155-180-GB1_AD
Plasmid#148544PurposeBacterial Expression of SpEDC1opt_155-180DepositorInsertSpEDC1opt_155-180
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HCV-Fluc_AC
Plasmid#148447PurposeMammalian Expression of FlucDepositorInsertFluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Rluc-6xbsMS2-random_AC
Plasmid#148431PurposeMammalian Expression of 6xbsMS2-randomDepositorInsert6xbsMS2-random
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1_C
Plasmid#146087PurposeMammalian Expression of HsTNRC6Ciso1DepositorInsertHsTNRC6Ciso1 (TNRC6C Human)
UseTagsExpressionMammalianMutationtwo silent and three non silent mutations, S337G,…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso3_C
Plasmid#146088PurposeMammalian Expression of HsTNRC6Biso3DepositorInsertHsTNRC6Biso3 (TNRC6B Human)
UseTagsExpressionMammalianMutationAA3-188 and AA residues242-1029 compared to the s…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C_C
Plasmid#146089PurposeMammalian Expression of HsTNRC6CDepositorInsertHsTNRC6C (TNRC6C Human)
UseTagsExpressionMammalianMutation3 silent mutations compared to the sequence of Hs…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH76
Plasmid#172144PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*)PromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH578
Plasmid#172162PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2_v2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH767
Plasmid#172172PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WT]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH857
Plasmid#172175PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH202
Plasmid#172150PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); 32A pylS variantPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH223
Plasmid#172151PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 34B_sub-pop5); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH224
Plasmid#172152PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 40C_sub-pop6); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH463
Plasmid#172155PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop1); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E122K, E178K, G188R)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH573
Plasmid#172157PurposepylBCD variant in pBAD backboneDepositorInsertPara (Untagged pylBCD); AraC
UseTagsExpressionBacterialMutationCodon-optimized Methanosarcina acetivorans pylBCDPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH574
Plasmid#172158PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K) PylC(E316D)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH575
Plasmid#172159PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop3); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH576
Plasmid#172160PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop4); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH19
Plasmid#172141PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH60
Plasmid#172142PurposeMicroscopy inclusion body assayDepositorInsertpCDF.CD
UseTagsExpressionBacterialMutationPylCD partial operon (PylB deleted to assess impa…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH77
Plasmid#172145PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, P83*, Y184*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH123
Plasmid#172146PurposeMicroscopy inclusion body assayDepositorInsertpCDF.B.(SD4)CD
UseTagsExpressionBacterialMutationPylBCD operon (RBS inserted for PylC)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#172147PurposeMicroscopy inclusion body assayDepositorInsertpCDF.SUMO-B.(SD4).CD
UseTagsExpressionBacterialMutationPylBCD operon (SUMO tag inserted for PylB; RBS in…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#2
Plasmid#163395PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#1
Plasmid#163394PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#3
Plasmid#163396PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1C-YFP-shSCR
Plasmid#139647PurposeReplacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.DepositorInsertshSCR
UseLentiviral and RNAiTagsExpressionMammalianMutationSee Depositor commentsPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBL-2
Plasmid#162769PurposeMinimal backbone with AmpRDepositorTypeEmpty backboneUseSynthetic Biology; Molecular cloningTagsExpressionMutationPromoternoAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only