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Plasmid#135474PurposeEncodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsertMarburg virus minigenome, eGFP reporter
UseTagsExpressionMammalianMutationNonePromoterT7Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
UseTagsExpressionMammalianMutationG299CPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-HSP70
Plasmid#163790PurposeExpresses mScarlet-Hsp70 in mammalian cellsDepositorInsertHSP70 (HSPA1A Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-1-adrenergic-receptor
Plasmid#14698DepositorInsertB1AR (ADRB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-trCIB1
Plasmid#64119PurposePhotoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1DepositorInsertdCas9-trCIB1 fusion
UseTagsHA and NLSExpressionMammalianMutationdCas9 (D10A H840A, catalytically inactive), trCIB…PromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 151-241
Plasmid#24390DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 151-241.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 243-450
Plasmid#24392DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 243-450.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-(151-241)
Plasmid#24393DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationAmino acids 151-241 are deleted. Delta CC mutant.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-SOX4
Plasmid#51304PurposeExpression of SOX4 after doxycycline administrationDepositorInsertSRY (sex determining region Y)-box 4 (SOX4 Human)
UseLentiviralTagsn/aExpressionMutationfull length ORFPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-2-adrenergic-receptor
Plasmid#14697DepositorInsertB2AR (ADRB2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BirA-P2A-mCherry
Plasmid#74056Purposeretroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion proteinDepositorInsertBirA biotin ligase
UseRetroviralTagsP2A-mCherryExpressionMammalianMutationPromoterMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-E
Plasmid#78852PurposeIntroduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsCas9-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorInsertSF2 (SRSF1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-LRPAP1
Plasmid#31327DepositorInsertlow density lipoprotein receptor-related protein associated (LRPAP1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only