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pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
Plasmid#216244PurposeExpress AtAFB2.F74A mutant with mCherry tag for AID2DepositorInsertAtAFB2.F74A-mCherry
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsmCherryExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-diLACCO1
Plasmid#208028PurposeControl biosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-42)
Plasmid#127151PurposeExpresses N-terminal cysteine Abeta(1-42)DepositorInsertAbeta(MC1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/avitag-apex2-v5-fkbp8
Plasmid#154460Purposeexpresses avitag-apex2-v5-fkbp8/biochemical assayDepositorInsertavitag-apex2-v5-fkbp8 (FKBP8 Human)
UseTagsExpressionMammalianMutationPromoterCMV-F, T7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
UseTagsExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST-DHFR Mm
Plasmid#83073PurposeExpresses the full length DHFR Mm in E.coliDepositorInsert2x DHFR (Dhfr Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-BATF
Plasmid#178451Purposedoxycycline-inducible expression of Human BATF in mammalian cellsDepositorInsertBATF (BATF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT K842M (Puro)
Plasmid#154291PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationK842M mutation (Catalytic Inactive)PromoterPGKAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A6
Plasmid#132196PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A6 (SLC6A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
mAC-POLR2A donor (Hygro)
Plasmid#124496PurposeA donor plasmid for tagging the N-terminus of human POLR2A with mAID-mCloverDepositorInsertPOLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
UseCRISPRTagsmAID-mCloverExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-POLR2A-CTD-mCherry
Plasmid#185011PurposeBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion ProteinDepositorInsertPOLR2A (Polr2a Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKO-2TO
Plasmid#86697PurposeTetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterH1 2TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EFIa-Id1-PGK-puro
Plasmid#107735PurposeExpresses Id1 in mammalian cellsDepositorInsertId1 (T Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
TH1204-pOCC120-FUS_opt(NheI)
Plasmid#221892PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTagsMBP-PreScission and mGFP-PreScission-6xHisExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)
Plasmid#223249PurposeMammalian expression of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221893PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-eLACCO2.1-NGR
Plasmid#208023PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only
