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Plasmid#114222PurposePOC12358, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-dDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_dFe
Plasmid#114223PurposePOC12359, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-eDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_aFq
Plasmid#114224PurposePOC12362, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_bFq
Plasmid#114225PurposePOC12363, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_cFq
Plasmid#114226PurposePOC12364, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_dFq
Plasmid#114227PurposePOC12365, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_eFq
Plasmid#114228PurposePOC12366, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type e-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet50K
Plasmid#118399Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationincorporation of oligonucleotide during cloningPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMPO 51
Plasmid#68358PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHRS11
Plasmid#39157PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDHRS11 (DHRS11 Human)
UseTagsHis TevExpressionBacterialMutationG99L100 -> AHM; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRGD-TcR
Plasmid#74110PurposePlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sitesDepositorInserttetA(C)
UseBacterial vector based on pmb1 repliconTagsExpressionMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorInsertChimsonR-mCherry
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978DepositorInsertflp
UseSynthetic BiologyTagsExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-CRY2-uTEV1Δ(220-242)
Plasmid#135452PurposeYeast expression of uTEV1Δ fused to CRY2 and mCherryDepositorInsertmCherry-CRY2-TEV1Δ(220-242)
UseTagsmCherryExpressionYeastMutationPromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GZnP3 in pcDNA3.1+
Plasmid#161738PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)DepositorInsertGZnP3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
GZnP2 in pcDNA3.1+
Plasmid#161739PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)DepositorInsertGZnP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only