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Plasmid#204471PurposeExpression plasmid for PE protein in bacteria.DepositorInsertPrime editor
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMusheLL
Plasmid#47023PurposeMouse PV L1 and L2, Bicistronic, too large to self-packageDepositorInsertMu L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PLE-IRES-mCherry
Plasmid#45766DepositorInsertesterase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP
Plasmid#85428PurposeLentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-12
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p006-Banana-Late
Plasmid#112251PurposeATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.DepositorInsertATF1 under osmY promoter
UseTagsExpressionBacterialMutationPromoterosmYAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
UseTags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-WT-VWF
Plasmid#124794PurposeExpresses multimeric human VWF with C-terminal c-Myc tagDepositorInsertVWF (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCT62
Plasmid#170391Purposeexpresses three sgRNA cassettesDepositorInsertPCT62-targetsite
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2
Plasmid#88917PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
TS181_pETNKI_his3C_pA-Dam
Plasmid#200721Purposeproducing pA-DamID methylation protein in bacteriaDepositorInsertHis3C-pA-Dam
UseTagsHisExpressionBacterialMutationcodon optimizedPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAD-F3-NPEST-iCRE-2A-mCherryPEST
Plasmid#99608PurposeSelf-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherryDepositorInsertsiCRE
mCherry
UseUnspecifiedTagsPESTExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-mphR
Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only