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6CW0 (cgd2_2690)
Plasmid#119965PurposeBacterial expression for structure determinationDepositorInsertcgd2_2690
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 1-105PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-L126X-N’His
Plasmid#117707Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationL126XPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q42L-N’His
Plasmid#117708Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ42LPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q123A-N’His
Plasmid#117709Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ123APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-D83G-N’His
Plasmid#117702Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD83GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-D101G-N’His
Plasmid#117704Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD101GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
5K29 (PP-BRD3)
Plasmid#101883PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
5TCM (PP-BRD3)
Plasmid#101888PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK545
Plasmid#74225PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationcontains a silent mutation in Ala63 codonPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK361
Plasmid#72403PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tagDepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK108
Plasmid#73312PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)DepositorInsertdelta-12 fatty acid acetylenase
UseTagsExpressionBacterialMutationsynthetic genePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK368
Plasmid#72407PurposeProduces Acetobacter aceti 1023 Est12DepositorInsertacyl-CoA thioesterase
UseTagsExpressionBacterialMutationadditional N-terminal 15 residues MSETYGTGQKEQHC,…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK692
Plasmid#71643PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges histidine-17 to tryptophan, serine-38 to …PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK653
Plasmid#71630PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to threonine, phenylalanine-79 …PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK654
Plasmid#71631PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to alanine, phenylalanine-79 to…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK655
Plasmid#71632PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to aspartate, phenylalanine-79 …PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK656
Plasmid#71633PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to valine, phenylalanine-79 to …PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK659
Plasmid#71634PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to cysteine, phenylalanine-79 t…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only
