Skip to main content
Addgene
Showing: 10281 - 10300 of 11049 results

  7. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    delta Tat
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

    Enlarge

  8. pQE60-mPU.1-ETS

    Plasmid
    #85733
    Purpose
    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4
    Depositor
    Gregory Poon
    Article
    Poon et al J Biol Chem. 2012 May 25;287(22):18297-3
    Insert
    ETS domain of transcription factor PU.1 (Spi1 Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85733-plasmid-map-sequence-id-175402

    Enlarge

  12. NMUR2-DuET

    Plasmid
    #213350
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NMUR2 (NMUR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213350-plasmid-map-sequence-id-430914

    Enlarge

  13. SSTR1-DuET

    Plasmid
    #213376
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR1 (SSTR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213376-plasmid-map-sequence-id-430732

    Enlarge

Showing: 10281 - 10300 of 11049 results