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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggtaC

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggAag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->gCtag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW213

Plasmid

#113235

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->Cgtag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW211

Plasmid

#113233

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->AACGA

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

H208A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggtTg

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

E10A

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-M-RAN-H2A2B

Plasmid

#106420

Purpose

M-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-H2A2B

Use

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PP322

Plasmid

#78943

Purpose

Expresses GFP. Figure 3. Strain 9.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP295

Plasmid

#78935

Purpose

Expresses GFP. Figure 3. Strain 1.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Use

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDA199

Plasmid

#74275

Purpose

pSTL1-dPSTRy(4,3)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRy(4,3)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB-Pinus-banksianaPHLS_CpcA

Plasmid

#74002

Purpose

Expresses the Pinus banksiana PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB-Picea-sitchensisPHLS_CpcA

Plasmid

#74001

Purpose

Expresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB-Abies-grandisPHLS_CpcA

Plasmid

#74000

Purpose

Expresses the Abies grandis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-5

Plasmid

#72737

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-18

Plasmid

#72750

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-17

Plasmid

#72749

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-16

Plasmid

#72748

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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