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pHAGE-PIK3CG-R130Q

Plasmid

#116581

Purpose

Lentiviral expression of PIK3CG R130Q

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Tags

Expression

Mutation

R130Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-S806F

Plasmid

#116583

Purpose

Lentiviral expression of PIK3CG S806F

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Tags

Expression

Mutation

S806F

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R604Gfs*4

Plasmid

#116560

Purpose

Lentiviral expression of PIK3CB R604Gfs*4

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Tags

Expression

Mutation

R604Gfs*4

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R604Pfs*29

Plasmid

#116561

Purpose

Lentiviral expression of PIK3CB R604Pfs*29

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Tags

Expression

Mutation

R604Pfs*29

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Tags

Expression

Plant

Mutation

Promoter

RCH1

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_PIK3CB_p.E497D

Plasmid

#81599

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E497D; Q759R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_p.A593V

Plasmid

#81602

Purpose

Gateway Donor vector containing PIK3CB ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A593V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_p.A1048V

Plasmid

#81533

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A1048V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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DGKZ gRNA (BRDN0001147837)

Plasmid

#77464

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKZ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKZ (DGKZ Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKZ gRNA (BRDN0001149311)

Plasmid

#77465

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKZ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKZ (DGKZ Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKZ gRNA (BRDN0001147025)

Plasmid

#77466

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKZ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKZ (DGKZ Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146432)

Plasmid

#77063

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146757)

Plasmid

#77064

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1 gRNA (BRDN0001147781)

Plasmid

#76758

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1 (ACVR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1 gRNA (BRDN0001144892)

Plasmid

#76759

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1 (ACVR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT-CA

Plasmid

#195576

Purpose

Expresses STAT3 constitutively active mutant

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

changed Alanine 662 to Cysteine and Asparagine 66…

Promoter

CMV enhancer and promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E545K

Plasmid

#116485

Purpose

Lentiviral expression of PIK3CA E545K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

E545K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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