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Plasmid#77548Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB2DepositorInsertEPHB2 (EPHB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4 gRNA (BRDN0001144879)
Plasmid#76352Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4 gRNA (BRDN0001148436)
Plasmid#76353Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD2 gRNA (BRDN0001145548)
Plasmid#77986Purpose3rd generation lentiviral gRNA plasmid targeting human BRD2DepositorInsertBRD2 (BRD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GP805
Plasmid#164545PurposeExpresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717RDepositorInsertSmoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationAll 21 cytoplamic Lysines of Smoothened changed t…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP804
Plasmid#164548PurposeExpresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717RDepositorInsertSmoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationAll sixteen Lysines in cytoplasmic C-terminal tai…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP803
Plasmid#164547PurposeExpresses Smo-FLAG K434, 444, 448RDepositorInsertSmoothened K434, 444, 448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationAll three Lysines in cytoplasmic loop three of Sm…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP802
Plasmid#164546PurposeExpresses Smo-FLAG K348, 360RDepositorInsertSmoothened K348, 360R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationBoth the Lysines in cytoplasmic loop two of Smoo…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp95bGW (B5)
Plasmid#74014Purposelentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and Psd95 shRNADepositorInsertPsd95 shRNA (Dlg4 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FHp5pUP95C3,5SGW (E5)
Plasmid#74035Purposelentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationPCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…PromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MKNK1_IDG-K
Plasmid#170593PurposeGateway destination clone of MKNK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-MKNK1 (MKNK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001145885)
Plasmid#80196Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-DYRK3-V5-miniTurbo_IDG-K
Plasmid#158190PurposeGateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertDYRK3-V5-miniTurbo (DYRK3 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationR293H- please see depositor commentsPromoterUbiquitinAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIN52 D5.4 gRNA
Plasmid#90734Purpose3rd generation lentiviral gRNA plasmid targeting human LIN52DepositorInsertLIN52 (Guide Designation D5.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P85(G376R)
Plasmid#40220DepositorInsertP85(G376R) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 376 to ArgininePromoterCMVAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS
Plasmid#106355PurposeCRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locusDepositorInsertMYOD1 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
ANLN A1.2 gRNA
Plasmid#90530Purpose3rd generation lentiviral gRNA plasmid targeting human ANLNDepositorInsertANLN (Guide Designation A1.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MORC2 ts1
Plasmid#115886PurposeMORC2 knockdownDepositorInsertMORC2 shRNA (MORC2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only