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148,365 results

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pET28a-gp32-H6

Plasmid

#163912

Purpose

Expresses T4 gp32 for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

gp32

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX_307

Plasmid

#41392

Purpose

Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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12XCSL-d1EGFP

Plasmid

#47684

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

d1EGFP

Expression

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-GRAB-HTR6-cilia

Plasmid

#189615

Purpose

Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6_GRAB

Use

AAV

Tags

Expression

Mutation

Promoter

Syn1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Has Service

Cloning Grade DNA

Insert

iRFP

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

Plasmid

#69625

Purpose

A FRET sensor for Galphai3 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2

Use

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Stat3 pcDNA3

Plasmid

#8706

Purpose

See Depositor Comments below for variant information.

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

* see comments

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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