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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.2.3

Plasmid

#216529

Purpose

Encodes SARS-CoV-2 spike for XBB.2.3 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.2.3 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) E484K (AAA)

Plasmid

#212352

Purpose

Encodes SARS-CoV-2 spike K417N, E4848K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, E4848K (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

K417N, E4848K

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA) N501Y (TAT)

Plasmid

#212354

Purpose

Encodes SARS-CoV-2 spike E484K, N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K, N501Y (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

E484K, N501Y

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaH69V70

Plasmid

#212336

Purpose

Encodes SARS-CoV-2 spike deltaH69, deltaH70 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike deltaH69, deltaH70 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

deltaH69, deltaH70

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

Plasmid

#223140

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_allW

Plasmid

#224252

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_allW (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

Plasmid

#223150

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

Plasmid

#223151

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

Plasmid

#223152

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tailless Control

Plasmid

#182718

Purpose

expresses a truncated force-insensitive DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

Insert

desmoglein-3 (Dsg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

TSmod inserted at G726, downstream C-terminal tai…

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-N SARS-CoV-2 Omicron spike protein

Plasmid

#180779

Purpose

Sars-CoV-2 variant protein

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

MAC-N SARS-CoV-2 Omicron spike protein

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

S371L, H69-V70-, S373P, S375F, G339D, N211-, N440…

Promoter

Available since

Sept. 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CAGGS-safb∆dd1-NLS-3XFLAG-V5

Plasmid

#196092

Purpose

Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Removed amino acids 96-285 (predicted disordered …

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-safb∆dd3-NLS-3XFLAG-V5

Plasmid

#196094

Purpose

Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Removed amino acids 638-925 (predicted disordered…

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-DD3-NLS-3XFLAG-V5

Plasmid

#196095

Purpose

Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Contains only AA 619-925 (NLS and predicted disor…

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR

Plasmid

#186744

Purpose

EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X

Plasmid

#147983

Purpose

Insect Expression of DmNot1_1-1717-del1156-1169

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-del1156-1169 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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