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pBE48

Plasmid

#174656

Purpose

CAPTURE method BAC origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMO9075

Plasmid

#117481

Purpose

empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

Depositor

Judy Wall

Article

Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

Insert

Aph(3')-IIa promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Aph(3')-IIa promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-CITRINE

Plasmid

#104839

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Citrine

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

Plasmid

#135459

Purpose

Mammalian expression of SPARK protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-p2A-HA-βarrestin2-uTEV1Δ

Use

Lentiviral

Tags

eGFP, HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3755

Plasmid

#154116

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::act-4 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C360A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pNM3724

Plasmid

#154112

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp7-Flag

Plasmid

#11815

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 (CASP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pCis mCherry-SS Himar1 A7

Plasmid

#84687

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLX208-beta-arrestin2-HA-TEV219

Plasmid

#104846

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-Luciferase

Plasmid

#104840

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLXMA_RK2 L2A

Plasmid

#153361

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pOSIP-KL (KanR, Lambda)

Plasmid

#45984

Depositor

Keith Shearwin, Drew Endy

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pAWP89

Plasmid

#61264

Purpose

IncP-based replicating vector expressing dTomato under tac promoter.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Insert

Ptac-dTomato

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV--Mouse Mdm2 wt-IRES-CFP

Plasmid

#180493

Purpose

Role of Mdm2 splicing variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

Mdm2 full length (wt)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_HaloTag7

Plasmid

#175527

Purpose

Strict cytosolic expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pOSIP-KP (KanR, Phi80)

Plasmid

#45986

Depositor

Keith Shearwin, Drew Endy

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge