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Plasmid#174791PurposeXenopus laevis WBP2NL with 3'HA tagDepositorInsertWBP2 N-terminal like (wbp2nl.L Frog)
UseTagsHAExpressionBacterialMutationaa#165, Y to H; aa# 287, D to HPromoterSP6Available sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJet-gR-X4ex2-DTS
Plasmid#177154PurposeDonor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4DepositorInsertMT-C34-pA with CXCR4 arms of homology
UsePcr cloningTagsExpressionMutationPromoternoneAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB_ROSA26_SpCas9_gRNAs
Plasmid#174703PurposePlasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.DepositorInsertSpCas9 dual gRNAs
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP
Plasmid#171838PurposeMammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationH85EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP
Plasmid#171839PurposeMammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK88EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP
Plasmid#171840PurposeMammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationI92EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP
Plasmid#171841PurposeMammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationC93EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP
Plasmid#171842PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR108EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP
Plasmid#171843PurposeMammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR125EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP
Plasmid#171844PurposeMammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK128EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nl
Plasmid#172463Purposeexpresses wild-type protein when injected into embryosDepositorInsertwbp2nl (wbp2nl.L Frog)
UseXenopus expressionTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585
Plasmid#174397PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585_S130A
Plasmid#174403PurposeBacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.DepositorInsertsarA
UseTagsExpressionBacterialMutationsarA with S130A mutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585
Plasmid#174391PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tagDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#1
Plasmid#170834PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only