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Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionTagsExpressionMutationPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM012_P5_ConR11
Plasmid#216437PurposeAllows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.DepositorInsertConnector R11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM011_P5_ConR10
Plasmid#216436PurposeAllows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.DepositorInsertConnector R10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM006_P1_ConL11
Plasmid#216431PurposeAllows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.DepositorInsertConnector L11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM005_P1_ConL10
Plasmid#216430PurposeAllows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.DepositorInsertConnector L10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-OptoSTIM1(CRY2clust)
Plasmid#184715PurposeOptoSTIM1(CRY2clust)DepositorInsertOptoSTIM1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG-Pcon-Scar
Plasmid#203678PurposepQFT-mNG with constitutively expressed mScarlet-IDepositorInsertsmNeonGreen
mScarlet-I
UseTagsExpressionBacterialMutationPromoterPQJ and Phyb18Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLL_CylM
Plasmid#208759PurposeExpresses modified cytolysin L (mCylLL) in E. coliDepositorInsertsCylLL
CylLM
UseTagsHisx6 TagExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLS_CylM
Plasmid#208760PurposeExpresses modified cytolysin S (mCylLS) in E. coliDepositorInsertsCylLS
CylLM
UseTagsHisx6 TagExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0013
Plasmid#205582PurposeNpuDnaE(C)_HYG(53-341)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0011
Plasmid#205580PurposeNpuDnaE(C)_KAN(193-265)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0010
Plasmid#205579PurposeKAN(1-192)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0009
Plasmid#205578PurposeNpuDnaE(C)_KAN(132-265)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0008
Plasmid#205577PurposeKAN(1-131)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0007
Plasmid#205576PurposeNpuDnaE(C)-Glucos(232-500)DepositorInsertNpuDnaE(C)-Glucosyltransferase
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only